Mouse B-cell Activating Factor Chemiluminescent Immunoassay Kit from Innovative Research is a highly sensitive Chemiluminescent Immunoassay for meauring B-cell Activating Factor in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests. This assay recognizes Mouse BAFF/CD257 in samples. No significant cross-reactivity or interference between Mouse BAFF/CD257 and analogues was observed. Innovative Research's B-cell Activating Factor Chemiluminescent Immunoassay kit uses the Sandwich-Chemiluminescent Immunoassay principle. The micro Chemiluminescent Immunoassay plate provided in this kit has been pre-coated with an antibody specific to Mouse BAFF/CD257. Standards or samples are added to the micro Chemiluminescent Immunoassay plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BAFF/CD257 and Avidin-Horseradish Peroxidase(HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BAFF/CD257, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BAFF/CD257. You can calculate the concentration of Mouse BAFF/CD257 in the samples by comparing the RLU value of the samples to the standard curve. Detection Target: B-cell Activating Factor Reactivity: Mouse Range: 15.63 - 1000 pg/mL Sensitivity: 9.38 pg/mL Storage Conditions: An unopened kit can be stored at 2-8 C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.